Techniques in Molecular Biology Book
International Book Distributing Co. | 2008 | ISBN-10: 8181891511, 1441649263 | 337 Pages | PDF | 12.7 MB
Completion of the human genome project is an important mile stone in the understanding of the complex processes involved in modern biology. It is a step towards understanding more precisely the function of each gene which may in turn lead in future to practice molecular and individualized medicine. Practice of molecular medicine is a two fold interaction of intensive basic and applied research. All the techniques which are being used in molecular biology are highly sophisticated and advanced. Moreover these techniques are unique for a set of hypothesis being proposed to answer a specific question. Various molecular techniques have application not only in the field of biotechnology forensic science, and pharmaceutical research but also have a wide potential in medical sciences. In this manual we have made an attempt to provide the basic back ground of each technique followed by detailed recipe and then how to carryout different experiments.
The topics dealt in this book are preparation of Nucleic Acids that includes Deoxyribose Nucleic Acid (DNA) Extraction, Ribose Nucleic Acid (RNA) Extraction and Plasmid DNA Extraction; Basic Molecular techniques including Polymerase Chain Reaction (PCR) and
Electrophoretic Techniques; Gene expression analysis using Reverse transcriptase PCR (RT-PCR); Molecular genotyping of Short Tandem Repeats (STR), Alu insertions; Mutation detection techniques including Restriction Fragment Length Polymorphism (RFLP), Amplification Refractory Mutation Detection System (ARMS) and Single Stranded Conformation Polymorphism (SSCP); Nucleic Acid Blotting: Dot Blotting and Southern Blotting; Latest molecular techniques like DNA Sequencing, Multiplex PCR and Automated DNA Fragment size analysis by Gene scanning; DNA Recombinant Technology and Microarray.
A separate chapter on a brief overview of all the statistical methods which can be used to analyze the biological data has been included. This book has been written keeping in mind the bench workers and also the researchers who are going to establish their own laboratories. We have tried to give point wise description of various techniques. Although every effort has been made to make this book as up to date as possible, there may still be some deficiencies and mistakes. The comments and suggestions from users are solicited for improvement.
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